Casp8 Knockout cell line （MLE-12）

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location： Home > Products > KO Cell Lines > Casp8 Knockout cell line （MLE-12）

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Casp8 Knockout cell line （MLE-12） Order

Casp8 Knockout cell line （MLE-12）
Catalog#
YKO-M242
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Casp8 Knockout cell line （MLE-12）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Casp8 Knockout cell line (MLE-12)
Morphology
Epithelial-like, adheren
Passage ratio
1：2-1：3
Species
Mouse
Gene Name
Casp8
Gene ID
12370
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Speciality Media
Target region
Knockout region: Exon 3 (based on transcript Casp8-204)
Validation: PCR+Sanger Sequencing
Casp8 Knockout cell line （MLE-12） Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MLE-12		 STR Info (Cell bank)
Cell Line:MLE-12
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.0156.39
【17】
4-2 19.3233.94
【19.3】
6-7 12.0334.52
【12】
19-2 12.0
1-2 13.0
7-1 29.0
8-1 15.0
1-1 10.0
3-2 14.0
2-1 9.0
15-3 20.3192.1
【20.3】
6-4 16.0290.93
【15.3】
13-1 15.2
11-2 15.0
17-2 15.0
12-1 20.0242
【20】
5-5 14.0335.92
【14】
X-1 26.0405.05
【25.3】
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Casp8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MLE-12 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Casp8 gene knockout Casp8 Knockout cell line (MLE-12) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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